Fig. 2.

Activin pathway regulates InR/TOR signaling in the body wall. Representative immunoblot images and quantification of pAKT1 and pS6K. (A) Phosphorylation of AKT1 at S505 and S6K at T398 sites are down- and upregulated by muscular expression of InR-RNAi and InR, respectively, suggesting that InR signaling positively regulates the phosphorylation at these sites. (B) Phosphorylation of AKT1 is increased, whereas the pS6K level is decreased in the larval body walls of babo and Smad2 mutants. (C) Resupply of Activin signaling in Smad2 muscle restores pAKT1 and pS6K levels close to those of the wild type. (D) Wandering larvae display the same pattern of alteration in the AKT1 and S6K phosphorylation in the larval body wall as foraging larvae. Values are mean±s.e.m. *P<0.05, **P<0.01 and ***P<0.001 from one-way ANOVA followed by Dunnett's test in which each genotype was compared with Mef2-Gal4/+ control (A) or wt (B-D). Additionally, unpaired t-tests were performed in C as indicated by lines; ^##P<0.01 and ^###P<0.001.