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. 2021 Jan 11;148(1):dev190868. doi: 10.1242/dev.190868

Fig. 6.

Fig. 6.

Z-disc number and muscle thickness are decoupled from Mhc level. (A) Overexpression of Pdk1 or S6kCA in Smad2 muscle rescues the muscle width but not the Z-disc number. (B) Overexpression of Pdk1 or S6kCA fails to restore the reduced thickness of Smad2 muscle. (C) Pdk1RNAi expression decreases the muscle width but not the Z-disc number. (D) Although the S6kRNAi and S6kCA profoundly affect the Mhc level in larval body wall tissue (Fig. 5E), they have no or little effect on Z-disc number of the muscle. However, the muscle width is significantly reduced by S6kRNAi and increased by S6kCA expression. (E) Representative images of muscles 6 and 7 of abdominal segment 2 stained with α-Actn antibody. (F) Z-disc number and relative width of the muscle expressing baboCA alone and together with Smad2RNAi or TorDN. (G) Representative wandering stage larvae expressing Smad2RNAi or baboCA in the muscle and a control larva. Values are mean±s.e.m. *P<0.05, **P<0.01 and ***P<0.001 from one-way ANOVA followed by Dunnett's test in which each genotype was compared with UAS-dicer2/+;Mef2-Gal4/+ control (D), Mef2-Gal4/+ control (F) or Mef2-Gal4/+ control in Smad2 mutant background (A,B). Unpaired t-tests were performed in C and F (indicated by lines); #P<0.05, ###P<0.001. Scale bar: 120 μm.