Fig. 6.

Chronic BPA or ARS suppresses eIF2α-dependent SG formation. (A) Schematic of the protocol for experiments in Figs 6B,C and 7. Cells were not treated (UN, white bars) or chronically treated for 6 days with 500 nM arsenite (AR, light grey bars), or 10 nM BPA (BP, dark grey bars). After chronic treatment protocol, acute stresses (100 μM arsenite, 400 μM BPA, or 0.2 M sodium chloride) were applied for 30 min (B) or 60 min (C), followed by fixation or processing for western blotting. An additional experimental replicate was treated for 60 min with acute stress and then recovered for 20-22 h before processing for western blotting or Coomassie stain (see Fig. S4). n=4; error bars are ±s.e.m.; *P<0.05, **P<0.01 by one-way ANOVA within acute treatment groups.