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. 2021 Jan 22;41(1):BSR20202298. doi: 10.1042/BSR20202298

Figure 3. The effect of silencing miR-193b-3p on shH19 inhibiting PDGF-BB-induced dedifferentiation and migration of HASMCs.

Figure 3

(A,B) The expressions of lncRNA H19 and miR-193b-3p in PDGF-BB, PDGF-BB + IC, PDGF-BB + shH19 + IC, PDGF-BB + shH19 + I groups were detected by qRT-PCR. GAPDH and U6 were used as internal reference genes. (C) After 72 h of incubation, the cell viability was detected by MTT method. (D,E) The cell migration of each group was detected by wound-healing assay. (F–H) qRT-PCR and Western blot were used to detect the levels of phenotypic differentiation markers α-SMA and SM22α, as well as the expressions of MMP-2 and MMP-9. GAPDH served as a control. Each experiment was repeated three times independently, and the results were expressed by the means ± SD. I: miR-193b-3p inhibitor. *P<0.05, **P <0.01, *** P<0.001 vs. PDGF-BB + IC; ^P<0.05, ^^P<0.01, ^^^P<0.001 vs. PDGF-BB + shH19 + IC.