Fig. 5.

ApoE deficiency up-regulated expression of DMT1 and down-regulated Fpn1 mRNA and protein in the small intestine.

The expression of DMT1 (A and B) and Fpn1 (A and C) protein at different ages (1, 2, 4 and 10 m = 1,2, 4, and 10 months) and DMT1 and Fpn1 protein (D: DMT1; E: Fpn1) and mRNA (F: DMT1; G: Fpn1) at 10-months old were measured in the small intestine of ApoE KO (KO) and wild-type (WT) mice by immunohistochemical analysis (D and E) or RT-PCR (F and G) as described in “Methods".

All n = 7 in WT; n = 10 in KO. Data were the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT mice.