Fig. 6.

Partial replenishment of ApoE partly reversed the iron related phenotype in ApoE−/− mice.

ApoE KO mice (10-months old) were treated with Ad-ApoE or Ad-Blank and then ApoE and FTL protein expression and iron contents in the liver and spleen were measured as described in “Methods”. A and B: a GFAAS measurement of total iron contents in the liver (A) and spleen (B) of Ad-ApoE-treated ApoE KO mice at 10-months old; C to F: Western blot analysis of ApoE (C and D) and FTL (E and F) protein in the liver (C and E) and spleen (D and F) of Ad-ApoE-treated ApoE KO mice at 10-months old. Data were the mean ± SEM (n = 7 in ApoE−/− + Ad-Blank and n = 10 in ApoE−/− + Ad-ApoE). **p < 0.01, ***p < 0.001 vs. ApoE^−/- + Ad-Blank in vivo.