Galanin inhibits apoptosis and hypertrophic response in vitro.
A,B Representative images and quantification of TUNEL-positive H9C2 cells exposed to hypoxia (H) or normoxia (N) for 16 h in presence or absence of galanin (10 nM). C Cell viability in normoxic or hypoxic H9C2 cells in presence or absence of 10 nM galanin measured by CellTiter-Glo assay. D,E Representative images and quantification of cross-sectional area, and F,G BNP and β-MHC mRNA expression levels in galanin-treated (10 nM) H9C2 cells exposed to 16 h of hypoxia (H) or normoxia (N). Data represents the mean ± SEM. *P < 0.05; ***P < 0.001 vs N. #P < 0.05; ##P < 0.01; ###P < 0.001 vs H. Data represents the mean ± SEM. Statistical analysis was carried out by one-way ANOVA.