Fig. 4.

Treatment with galanin prevents cardiac hypertrophy in I/R-remodelled hearts.

A Histological analysis of cardiac tissue stained with H&E (the first row) and WGA (the second row) from vehicle- or galanin (Gal)-treated mice subjected to sham surgery or myocardial ischemia for 30 min followed by reperfusion for 24 h. Galanin (0,7 μg/kg, i. v.) or vehicle (PBS) was injected at 15 min of reperfusion. B Comparison of the average cross-sectional area of cardiomyocytes from A, n = 6–8 mice per group. C,D Myocardial β-MHC and ɑ-MHC mRNA expression levels in vehicle or galanin-treated mice after 24 h of I/R, n = 4–5 mice per group. E Myocardium ischemic infarct size as percentage of the area at risk (AAR) in I/R and Gal-treated mice. Data represents the mean ± SEM. **P < 0.01; ***P < 0.001 vs Sham (S). #P < 0.05; ##P < 0.01; ###P < 0.001 vs I/R. Statistical analysis was carried out by one-way ANOVA. For E statistical analysis was carried out by Student's two-tailed t-test.