Fig. 6.

Galanin calibrates cardiometabolic reprogramming in I/R-remodelled hearts.

A Electron micrographs of hearts from vehicle- or galanin (Gal)-treated mice subjected to sham surgery or myocardial ischemia for 30 min followed by reperfusion for 24 h. Cardiac tissues were harvested for examination of myocardial ultrastructure by transmission electron microscopy (TEM). (M), mitochondria; (S), sarcomere, (*), damaged mitochondria, (∧), disrupted myofibrils. B,C Representative images of cardiac sections stained with MitoSox Red specific mROS indicator and quantification of MitoSox Red-positive stained tissue. D Myocardial SOD2 mRNA levels in vehicle- or galanin-treated mice subjected to 24 h of I/R or sham, n = 5–7 mice per group. E,F Palmitate oxidation and glucose oxidation in I/R-challenged hearts treated with vehicle or galanin. G Myocardial TFAM mRNA levels in vehicle- or galanin-treated mice subjected to 24 h of I/R or sham, n = 5–6 mice per group. Data are expressed as the mean ± SEM. **P < 0.01; ***P < 0.001 vs Sham (S). #P < 0.05; ##P < 0.01; ###P < 0.001 vs I/R. Statistical analysis was carried out by one-way ANOVA. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)