Figure 3.

CRISPR/Cas9 105 bp deletion of rs4841132 region increases glycogen, PPP1R3B RNA, and decreases LOC157273 RNA in HuH-7 cells. (A) Fold-change in glycogen in μg/mg protein. (B) Quantitative PCR of PPP1R3B and LOC157273 normalized to wild-type (WT) control (black). Control, no stimulation of cells (circles); hetero: heterozygously deleted rs4841132 region (blue); homo, homozygously deleted rs4841132 region (red); insulin, cells treated with 30 μM insulin (squares); OA, cells treated with 200 μM oleic acid (triangles). *P < 0.05; **P < 0.01.