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. 2020 Nov 24;106(2):372–387. doi: 10.1210/clinem/dgaa855

Figure 4.

Figure 4.

LOC157273 overexpression and knockdown decreases and increases glycogen and has variable effects on PPP1R3B RNA levels in Huh-7 cells. Overexpression of vector only or vector with LOC157273. (A) Fold change in glycogen in μg/mg protein and (B) quantitative PCR of PPP1R3B and LOC157273 normalized to vector control. Antisense oligonucleotide knockdown of LOC157273. (C) Fold-change in glycogen in μg/mg protein and (D) quantitative PCR of PPP1R3B and LOC157273 normalized to scrambled control. ASO, cells exposed to LOC157273 antisense oligonucleotides; control, no stimulation of cells (circles); insulin, cells treated with 30 μM insulin (squares); LOC, cells expressing LOC157273; OA; cells treated with 200uM oleic acid (triangles); scrambled: cells exposed to scrambled oligonucleotide; vector, cells expressing vector. *P < 0.05; **P < 0.01.