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LAT1 and CAT1 siRNA knockdown effect on (A) mRNA expression and (B) [3H]l-tryptophan uptake by TR-BBB cell lines. The siRNA transfection was carried out for 48 h, followed by mRNA expression and uptake study at 37 °C for 5 min. Each point represents the mean ± SEM (n = 4). ** p < 0.01 indicates a significant difference from the control.
