Figure 4.

Eicosanoids produced by activated WT BM-Macs. Analysis by LC-MS/MS of cell-free supernatants obtained from WT BM-Macs unstimulated (U) and stimulated for 24 h with IL-4 or LPS+IFNγ. Shown is mean and standard error of eicosanoids produced by WT BM-Macs unstimulated (U, Purple bars) or activated with IL-4 (Red bars) or LPS+IFNγ (Blue bars). Eicosanoids were identified based on matching chromatographic retention times (RTs), fragmentation patterns, and six characteristic and diagnostic ions. Cyclooxygenase (Cox), Lipoxygenase (LOX) Cytochrome P450 (CYP), non-enzymatically (n.e.), Thromboxane B₂ (TxB₂), Prostaglandin E₂ (PGE₂), prostaglandin D₂ (PGD₂), 13,14-dihydro-15-keto prostaglandin D₂ (dhk PGD₂), 20-Carboxy-arachidonic acid (20CooH AA), 12-Hydroxyheptadecatrenoic acid (12-HHTrE), Prostaglandin F metabolite (PGFM), Hepoxilin B3 (HXB3), HODE (Hydroxy-octadecadienoic acid), EpHOME (epoxy-octadecenoic acid), HETrE (hydroxy-eicosatrienoic acid), HDoHE (hydroxy-docosahexaenoic acid), DiHDPA (dihydroxydocosa-pentaenoic acid), DiHETrE (dihydroxy-eicosatrienoic acid), HEPE (Hydroxy-eicosapentaenoic acid), HETE (Hydroxy-eicosatetraenoic acid), HPETE (Hydroperoxy-eicosatetraenoic acid), arachidonic acid (AA), LA (linoleic acid), DGLA (Dihomo-gamma linolenic acid) EPA (eicosapentaenoic acid), DHA (docosahexaenoic acid), AdA (Adrenic acid). ^# p < 0.0001, ** p < 0.001 * p < 0.05 by two-way ANOVA with Dunnett’s correction for multiple comparisons of three independent determinations.