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. 2020 Dec 29;11(1):25. doi: 10.3390/biom11010025

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Contribution of Pla2g5 to eicosanoids produced by activated Macs. Analysis by LC-MS/MS of cell-free supernatants obtained from (a–c) WT and Pla2g5-null BM-Macs unstimulated (U) and stimulated for 24 h with IL-4 or LPS+IFNγ or (d) 20CooH AA measured in the supernatants collected from vector- and PLA2G5-siRNA transfected human monocyte-derived macrophages (h-Macs) stimulated with IL-4. Shown are (a) heatmap (b) volcano plots of mean values (c,d) graphs of mean and standard error of eicosanoids produced. ^# p < 0.0001, * p < 0.05 by two-way ANOVA with Sidak’s (c) correction for multiple comparisons or t-Test (d) of three independent determinations.