Figure 8.

Flow cytometric analysis of myeloid cells in gut-draining and eye-draining lymph nodes (mLN and cLN, respectively) at day 28 post-induction. Live EcN treatment decreased the proportion of iNOS+ monocytes/macrophages in mLN but not in cLN. Data are pooled from 2 independent experiments (n = 11 per group in total) and differences were quantified by unpaired Mann Whitney test; * p < 0.05. The monocytes/macrophages are defined as live cells CD45⁺CD11c⁻B220⁻CD3⁻CD49b⁻Ly⁻6G⁻SSC^lo, neutrophils are defined as live cells CD45⁺CD11c⁻B220⁻CD3⁻CD49b⁻Ly⁻6G⁺ and B cells are described as live cells CD45⁺CD11c⁻B220⁺, as described earlier [47].