Figure 1.

The antifungal activity of ethylenediaminetetraacetic acid disodium salt (EDTANa)_(n) against Fusarium graminearum. (A) The influence of sodium ion on EDTA effects. Conidia of F. graminearum were grown in SNA (1 g L⁻¹ KH₂PO₄, 1.0 g L⁻¹ KNO₃, 0.5 g L⁻¹ MgSO_4˙7H₂O, 0.5 g L⁻¹ KC1, 0.2 g L⁻¹ glucose, 0.2 g L⁻¹ sucrose) medium with 0.15 mM EDTANa_(n) (n = 0, 2, 3 or 4; pH 4.2) and were photographed after 24 h of cultivation. The effect of sodium ions was assayed by replacing EDTANa_(n) with NaCl and was used as the second control group. Each right panel is an enlarged view of the area in the black box in the left panel. The arrows indicate conglobate structures after 24 h in response to EDTANa_(n). The experiment was repeated three times with the same patterns. (B) Sensitivities of F. graminearum to EDTANa₂. The measurement was performed on minimal medium (MM) after 4 d at 25 °C. Photos were taken 3 days after incubation. The experiment was repeated three times with the same patterns.