Figure 6.

Chitin synthase activity and chitin content assay of Fusarium graminearum. (A) Chitin synthase activity was measured following (¹⁴C) GlcNAc incorporation into the filter-retainable polymer in the presence of Mg²⁺ or Mn²⁺. Data are expressed as nmol of GlcNAc incorporated per hour per mg of protein. (B) Mycelia were treated with EDTANa₂ or EDTANa₂ with additional cations. EDTANa₂ (0.15 mM) was saturated by adding Mg²⁺, Ca²⁺ or Mn²⁺ (0.45 mM) to SNA media. Data are represented as the means ± SD of three biological replicates (* indicates significant differences at p < 0.05 compared to CK).