Table 1.

Primers used for the amplification of full-length cDNAs of JYMaV RNAs from sample HZ and RT-PCR detection for JYMaV in jujube samples.

RNA	Primer	Sequence a (5′–3′)	Position	Product Size
			(nt)	(bp)
Amplification of full-length cDNAs of JYMaV
RNA1	5H	CTCAGCAGTAGTGTTCTCC	1–13	1066
	1087R	AGTGCTATCATTCCACAGACC	1067–1087
	690F	GACCCATTGCCTCTATGTTC	690–708	1539
	2228R	AAGATGTATACAATAAGATC	2209–2228
	2072F	CTACTGATCCTTTGCCATTCC	2072–2092	1303
	3374R	GCTCTGCCAAATTAATGTCTG	3354–3374
	AF	GATGCATCDAAATGGTCWGC	3283–3370	390
	CR	ATCATCWGARTGHACCAT	3653–3672
	3438F	GACTATTTGTGGTATAATTC	3438–3457	1897
	5534R	CAGACAACTGATCCTAACAC	5315–5334
	5199F	GGAGCTGGTCGAACTCTATG	5199–5218	1533
	6712R	GGCATGACATGATGATAGAT	6712–6731
	6534F	GTTCTAGATCTGTCTTCATGGT	6534–6555	627
	3C	CTCAGCAGTAGTGAACTCC	7148–7160
RNA2–6	5H	CTCAGCAGTAGTGTTCTCC	5′ end	-
	3C	CTCAGCAGTAGTGAACTCC	3′ end	-
5′RACE	Outer	CATGGCTACATGCTGACAGCCTA	-	652
	R3–5R1	CTTGCTTGTGGATTTGGTGAAG	597–618
	Inner	CGCGGATCCACAGCCTACTGATGATCAGTCGATG	-	435
	R3–5R2	AGGTTATAGCAGATCTTGGGAC	389–410
3′RACE	Outer	TACCGTCGTTCCACTAGTGATTT	-	483
	R3–3F1	CGCACCTGGCTTAACCTTAG	778–797
	Inner	CGCGGATCCTCCACTAGTGATTTCACTATAGG	-	290
	R3–3F2	GGGATACTGTTGCCAAGTTGAG	952–971
RT-PCR detection for JYMaV
RNA3	3-F	CTTCACCAAATCCACAAGCAAG	597–618	530
	3-R	CACATCACAGAAAAATGAGACC	1105–1126
RNA4	4-F	GAGCAGTCCACATTAACTAAG	887–907	357
	4-R	GGAATCTGGATCAAAGAACAC	1223–1243
RNA5	5-F	GCCCTTATTCCTATTCTCATCA	744–765	346
	5-R	ATCAAGTTCGAGAAGTGTGG	1070–1089
RNA6	6-F	CAAGCATAGCAATTGATGAC	387–406	431
	6-R	ACTTGATCTGTGGACTGAAC	798–817

^a D: G/A/T, W: A/T, R: A/G, H: A/T/C, V: G/A/C, Y: C/T. The artificially added nucleotides were underlined.