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PCR analysis of the transgenic aspen lines after 6 months of cryostorage in LN for the presence of bar and sp-Xeg recombinant genes; Bar gene (expected amplicon size ~310 bp), sp-Xeg (expected amplicon size ~720 bp); M—DNA length marker 100 bp (Evrogen); H2O—negative reaction control, pBI-Xeg and pBIBar—plasmid DNA (positive control); Pt, f2 and PtV22—non-transgenic lines; transgenic lines: PtXIBar9a, PtXIBar14a, f2XIBar5a, PtV22XBar2a, Xeg-1-1a, Xeg-1-1c, Xeg-2-1b, Xeg-2-3b.
