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. 2020 Dec 23;13(1):15. doi: 10.3390/v13010015

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) SDS-PAGE gel from gel-filtration purification of gp11. Lane 1: starting material, concentrated protein from the metal-affinity column that was loaded onto the S200 column. Lane 2: molecular weight marker, with weights indicated in kilodaltons. Lanes 3–8: Sequential, 1.4 mL elution fractions of the single gp11 peak from the S200 column. Fractions 42 (58.8 mL)–47 (65.8 mL). (b)Typical elution profile from a Superdex 200 (16/60) gel filtration column for gp11-containing fractions combined after metal-affinity purification. Magenta overlay is the elution profile of a standard protein mixture over the same flow rate conditions. The standards are (A) thyroglobulin (670 kDa); (B) γ-globulin (158 kDa); (C) ovalbumin (44 kDa); (D) myoglobin (17 kDa); and (E) vitamin B12 (1.35 kDa).