Figure 1.

Metabolic and biomass profiles of hard tissue (HT) and soft tissue (ST) biofilms plus or minus Candida albicans. Biofilms were grown in 96 well microtiter plates. Metabolic activity (A) was assessed using Alamar blue™ rezasurin dye to determine respiration rates, and biomass (B) was assessed using crystal violet as a disclosing stain to interpret differences in biofilm formation. Biofilms were grown and assayed in triplicate or quadruplicate on three separate occasions (a total of n = 11 biofilms from three separate experiments). Data represented as mean ± SD. Statistical analysis performed using an unpaired Mann–Whitney t-test to compare differences in mean (*** p < 0.001).