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. 2020 Dec 28;9(1):59. doi: 10.3390/microorganisms9010059

Figure 5.

Figure 5

Treatment outcomes of hard and soft tissue biofilms with or without C. albicans. After maturation, biofilms were treated with 0.2% CHX, 4% EDTA, 1% KI or 40 µg/mL MCZ for 10 min at room temperature. Alamar blue™ dye was used to assess metabolic activity was calculated against an untreated control (HT biofilm (A) and ST biofilm (C)). Data represent mean ± SD, carried out in triplicate in three independent experiments. A Mann–Whitney t test was carried out on the raw metabolic activity data to assess significant differences between untreated (UT) and treated biofilms (## p < 0.01, ### p < 0.001). Following metabolic assessment, biofilms were dried overnight at room temperature and biomass was determined via crystal violet staining (HT biofilm (B) and ST biofilm (D)). A dotted black line has been drawn to illustrate the average results of the UT control—C. albicans, and a black solid line entered to show the UT control + C. albicans. Data represent mean ± SD, carried out in triplicate or quadruplicate on three independent experiments (a total of 11 replicates from 3 separate experiments). A Mann–Whitney t test was used to compare significant differences between UT biofilms and treated biofilms (# p < 0.05, ### p < 0.001, *** p < 0.001).