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. 2020 Dec 25;10(1):26. doi: 10.3390/cells10010026

Figure 2.

Figure 2

RAW264.7 cells following indirect co-culture with ASCs in a Transwell system. (A) Representative images of RAW264.7 morphology following either LnASC or ObASC co-culture for 48-h. (B) Changes in anti-inflammatory gene expression of RAW264.7 cells following 48-h co-culture relative to untreated controls. (C) Changes in pro-inflammatory gene expression of RAW264.7 cells following 48-h co-culture relative to untreated controls. (D) Griess assay determination of nitrite concentration of 48-h conditioned media following 48-h co-culture relative to untreated controls. Values are presented as means (N = 3) ± SD of three independent experiments using one-way ANOVA followed by Dunnett’s multiple comparisons test. Statistical differences between the means are marked with ** p < 0.01, *** p < 0.001. Abbreviations: Arg1, arginase 1; Mrc1, mannose receptor C-type 1; IL-10, interleukin 10; iNOS, inducible nitric oxide synthase; IL-1β, interleukin 1 beta; TNFα, tumor necrosis factor alpha.