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. 2020 Dec 26;13(1):51. doi: 10.3390/nu13010051

Figure 1.

Figure 1

Neutrophils incubated with zinc release less neutrophil extracellular traps (NETs). (a–e,h) Neutrophils were isolated from peripheral blood of healthy donors. (f,g) HL-60 cells were differentiated with dimethylformamide into granulocyte-like cells. (a–e,h) Cells were incubated with or without 10 µM zinc and stimulated with (a,f,h) 100 nM phorbol 12-myristate 13-acetate (PMA), (b,g,h) 4 µM calcium ionophore A23187 (CI), (c,h) 10 µg/mL lipopolysaccharide isolated from P. aeruginosa (LPS PA), (d,h) 2 µg/mL lipopolysaccharide isolated from E. coli (LPS E. coli) or (e,h) 2.5 µM platelet activating factor (PAF). NETs release was analyzed microscopically and released DNA was quantified fluorometrically. Data are shown as means + SEM and were analyzed by one-way ANOVA with post hoc Dunn’s test (a,b), Holm–Sidak’s test (g), Tukey’s test (f), or Sidak’s test (c–e); (a–c) n = 6, (f,g) n = 3, * (p ≤ 0.05), ** (p ≤ 0.01). MPO myeloperoxidase; (h) scale bar = 50 um.