Skip to main content
. 2020 Dec 26;13(1):51. doi: 10.3390/nu13010051

Figure 3.

Figure 3

Zinc affects degranulation and the number of bacteria but does not alter phagocytosis. Neutrophils were incubated with or without 10 µM zinc and (a) primed with 25 ng/mL GM-CSF, incubated with 5 µg/mL cytochalasin B, 10 nM C5a, immunolabeled and analyzed by flow cytometry; (b) incubated with E. coli bioparticles and analysis of the percentage of cells phagocyting was analyzed by flow cytometry; (c) incubated with alive E. coli at the multiplicity of infection 10:1 (E. coli: neutrophils) for 3 h and the number of colonies of survived bacteria from the collected supernatants were counted. One-way ANOVA with post hoc (a) Sidak’s test, (b) Holm–Sidak’s test, (c) Kruskal–Wallis test. The means + SEM are shown; (a) n = 3, (b) n = 6, (c) n = 4; * (p ≤ 0.05).