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. 2020 Dec 26;13(1):51. doi: 10.3390/nu13010051

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Neutrophils of mice fed low-zinc content diet present decreased NETs release. Mice fed with a low-zinc and high-zinc content diet were sacrificed, and bone marrow neutrophils were isolated. (a,b) NETs release was stimulated with 4 µM CI, 10 µM PAF, 20 µg/mL LPS E. coli, 40 µg/mL LPS P. aeruginosa. Next, NETs release was analyzed by (a) fluorometry and (b) fluorescent microscopy. (c) Degranulation of CD63 was analyzed by flow cytometry. (d) ROS production was analyzed by fluorometry. (e) Percentage of phagocyting cells was analyzed by flow cytometry. (a,c,d) Two-way ANOVA with post hoc Dunnett’s test. (e) One-way ANOVA with post hoc Dunnett’s test. The means (a,c,e) + SEM; (d) ± SEM; (a,c,e) with individual values are shown; n = 6; * (p ≤ 0.05). NE: neutrophil elastase.