Partial least squares discriminant analysis (PLS-DA) scores plots of the ruminal metabolome of the beef steers fed diets supplemented with direct-fed microbials containing multiple microbial species and their fermentation products. CON = control; PROB = a blend of S. cerevisiae, Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and L. casei, and their fermentation products fed at 19 g/steer/day (PMI, Arden Hills, MN); SYNB = a blend of live S. cerevisiae and the fermentation products of S. cerevisiae, Enterococcus lactis, Bacillus licheniformis, and Bacillus subtilis fed at 28 g/steer/day PMI, Arden Hills, MN).