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. 2020 Dec 29;10(1):25. doi: 10.3390/antiox10010025

Figure 4.

Figure 4

Chronic sublethal oxidative insult induces senescence in ARPE-19 cells. (A) Selected networks of proteins showing down-regulation in H2O2-treated cells and involved in extracellular matrix organization and assembly. Node color is related to the log2 fold change difference in expression between H2O2 and Ctr. Ctr and H2O2 treated cells were seeded on coverslips H2O2-free medium and grown in for 72 h before processing; (B) Morphological changes of plasma membranes in live cells assessed by CellMask Red staining; magnification 20×; (C) Expression and distribution of ZO-1 determined by immunofluorescence analysis. Magnification 100×; (D) SA-β-Gal activity assay. Senescent cells are stained in blue, magnification 10×. In (BD) the images are representative of one out of three separate experiments.