Table 4.
PANTHER overrepresentation test of GO molecular function using tobacco and Arabidopsis homologs inferred from N. benthamiana NSs-interacting proteins. A total of 61,238 proteins (GO Ontology database, doi:10.5281/zenodo.4033054) were included in the tobacco reference list. All other test parameters are the same as those in Table 3.
| GO Molecular Function Complete | Tobacco Reference # |
NSs-Interacting Proteins | ||||
|---|---|---|---|---|---|---|
| # | Expected | Fold Enrichment | +/− | p Value | ||
| Unfolded protein binding | 284 | 4 | 0.16 | 24.64 | + | 4.15 × 10−2 |
| Binding | 21,517 | 26 | 12.30 | 2.11 | + | 5.53 × 10−3 |
| ATP binding | 4591 | 12 | 2.62 | 4.57 | + | 9.61 × 10−3 |
| Adenyl ribonucleotide binding | 4708 | 12 | 2.69 | 4.46 | + | 1.24 × 10−2 |
| Adenyl nucleotide binding | 4734 | 12 | 2.71 | 4.44 | + | 1.32 × 10−2 |
| Purine nucleotide binding | 5258 | 13 | 3.01 | 4.33 | + | 6.22 × 10−3 |
| Nucleotide binding | 5870 | 16 | 3.35 | 4.77 | + | 6.52 × 10−5 |
| Small molecule binding | 6512 | 17 | 3.72 | 4.57 | + | 3.66 × 10−5 |
| Nucleoside phosphate binding | 5870 | 16 | 3.35 | 4.77 | + | 6.52 × 10−5 |
| Purine ribonucleotide binding | 5217 | 13 | 2.98 | 4.36 | + | 5.70 × 10−3 |
| Ribonucleotide binding | 5285 | 14 | 3.02 | 4.63 | + | 9.62 × 10−4 |
| Carbohydrate derivative binding | 5332 | 14 | 3.05 | 4.59 | + | 1.07 × 10−3 |
| Purine ribonucleoside triphosphate binding | 5100 | 13 | 2.91 | 4.46 | + | 4.43 × 10−3 |
| Anion binding | 6438 | 16 | 3.68 | 4.35 | + | 2.38 × 10−4 |
| Ion binding | 11,853 | 20 | 6.77 | 2.95 | + | 1.59 × 10−3 |
| Unclassified | 26,668 | 2 | 15.24 | 0.13 | − | 0.00 |
| GO Molecular Function Complete |
Arabidopsis
Reference # |
NSs-Interacting Proteins | ||||
| # | Expected | Fold Enrichment | +/− | p Value | ||
| Cysteine-type endopeptidase activity | 72 | 4 | 0.11 | 36.26 | + | 9.91 × 10−3 |
| Cysteine-type peptidase activity | 102 | 4 | 0.16 | 25.60 | + | 3.71 × 10−2 |
| Catalytic activity | 8305 | 27 | 12.72 | 2.12 | + | 1.06 × 10−2 |
| Cation binding | 1647 | 11 | 2.52 | 4.36 | + | 5.00 × 10−2 |
| Ion binding | 3071 | 16 | 4.70 | 3.40 | + | 1.04 × 10−2 |
| Binding | 9721 | 31 | 14.89 | 2.08 | + | 1.26 × 10−3 |
| Protein binding | 5109 | 23 | 7.83 | 2.94 | + | 3.25 × 10−4 |
| Unclassified | 5502 | 1 | 8.43 | 0.12 | − | 0.00 |
Tobacco reference #: number of proteins that are classified in the category out of 61,238 Nicotiana tabacum reference proteins. All other column descriptions are the same as those in Table 3.