Figure 5.
Effect of different sugar concentrations and pH changes on GLA accumulation and relative expression of Acgox gene in vitro. Solid SGM media was supplemented with different sugar concentrations (glucose/fructose at a ratio of 2.33), and inoculated with 100 µL of a 106 spores/mL suspension. (a) Acgox relative expression, and (b) GLA accumulation were analyzed 6 days post-inoculation. Relative expression was normalized using β-tubulin as an internal control. The expression of gox gene under a sugar concentration of 1% at pH 3 was normalized as 1.0. Error bars represent a standard error of three independent biological replicates. Different letters above the columns indicate statistically significant differences at p < 0.05, as determined using the Tukey’s honest significant difference test.