Table 5.
Real-time PCR primer sequences 1.
| Target Gene | Primer Sequence, Forward (5′→3′) | Primer Sequence, Reverse (5′→3′) | Temperature (°C) | Accession Number |
|---|---|---|---|---|
| ZO-1 | CGGTGTCTTCGTAGTCGG | CAGTTGGTTTGGGTTTCAG | 59.4 | KJ000055 |
| Occludin | TATCTGTATCACTACTGCGTCG | CATTCACCCAATCCTCCA | 59.4 | KF193855 |
| ZO-2b | TACAGCGGGACTCTAAAATGG | TCACACGGTCGTTCTCAAAG | 60.3 | KM112095 |
| Claudin-b | GAGGGAATCTGGATGAGC | ATGGCAATGATGGTGAGA | 57.0 | KF193860 |
| JAM-A | ACTGTGAGGTGCTTGGAA | CTGTTGTGACTGAAGAAGGA | 61.4 | KY780630 |
| Claudin-c | GAGGGAATCTGGATGAGC | CTGTTATGAAAGCGGCAC | 59.4 | KF193859 |
| RhoA | GCAGGACAAGAGGACTATG | GTGTTCATCATTCCGTAGGT | 63.3 | MN661351 |
| Claudin-f | GCTGGAGTTGCCTGTCTTATTC | ACCAATCTCCCTCTTTTGTGTC | 57.1 | KM112097 |
| Claudin-3c | ATCACTCGGGACTTCTA | CAGCAAACCCAATGTAG | 57.0 | KF193858 |
| ROCK | AGTCCAAGTCTGCTGCTA | CCTCTCCTTCTGCTTCATC | 63.3 | KY780630 |
| Claudin-7a | ACTTACCAGGGACTGTGGATGT | CACTATCATCAAAGCACGGGT | 59.3 | KT625604 |
| Claudin-7b | CTAACTGTGGTGGTGATGAC | AACAATGCTACAAAGGGCTG | 59.3 | KT445866 |
| Claudin-11 | TCTCAACTGCTCTGTATCACTGC | TTTCTGGTTCACTTCCGAGG | 62.3 | KT445867 |
| Nectin | GCCAGTGACCAAGATGAC | ACAGTGCCATTCGGATTG | 61.4 | MN661350 |
| Claudin-12 | CCCTGAAGTGCCCACAA | GCGTATGTCACGGGAGAA | 55.4 | KF998571 |
| Claudin-15a | TGCTTTATTTCTTGGCTTTC | CTCGTACAGGGTTGAGGTG | 59.0 | KF193857 |
| Claudin-15b | AGTGTTCTAAGATAGGAGGGGAG | AGCCCTTCTCCGATTTCAT | 62.3 | KT757304 |
| Afadin | CCTGTGCTCACACTACTG | GTCGTTGCCTGGACTATG | 61.4 | MN661352 |
| E-cadherin | GACTGTAACGCTGAAGAGA | CTGTGGAGAGGAGATGTTC | 61.4 | MN661354 |
| α-catenin | GCAATCTTCTCTCCTTTATCC | ACTTGTGAACTCCAGCAAT | 61.4 | HQ338751 |
| β-catenin | GTCTGCTTGCCATCTTCA | CAGGTTGTGTAGAGTCGTAA | 64.5 | MN661349 |
| MLCK | GAAGGTCAGGGCATCTCA | GGGTCGGGCTTATCTACT | 53.0 | KM279719 |
| NMII | AGCCAACTCGTCAATGTC | CCTTGGAATACTTCTCTGTCT | 61.4 | MN661353 |
| β-actin | GGCTGTGCTGTCCCTGTA | GGGCATAACCCTCGTAGAT | 61.4 | M25013 |
1 ZO, zonula occludins; MLCK, myosin light chain kinase; JAM-A, junctional adhesion molecule-A; NMII, non-muscle myosin II; ROCK, Rho associated protein kinase; RhoA, a small Rho GTPase protein.