Figure 4.
Comparison of 2DE gel protein patterns of three MRRA clinical isolates with and without tigecycline treatment. In total, 25 μg of the protein extract of each isolate was separated on 2D gels, using IPG strips (pI 4–7). Protein spots were stained with silver stain and scanned using Densitometer GS-800 Mode Imager. Red circles in each gel indicate protein spots of MRSA isolates before and after treatment with 0.5 tigecycline that were separated on 2D gels. These were then calculated by using PDQuest analyses software.
