Table 2.
Sequences of oligonucleotide primers used for qPCR.
| Genes | Nucleotide Sequence of Primers (5′-3′) | Accession Numbers | Annealing Temperature | Amplicon Size (bp) |
|---|---|---|---|---|
| icaA | 5-GAGGTAAAGCCAACGCACTC-3 | AF086783 | * 60 | 151 |
| 5-CCTGTAACCGCACCAAGTTT-3 | ||||
| icaD | 5-ACCCAACGCTAAAATCATCG-3 | AF086783 | 60 | 211 |
| 5-GCGAAAATGCCCATAGTTTC-3 | ||||
| icaB | 5-ATACCGGCGACTGGGTTTAT-3 | AF086783 | 60 | 140 |
| 5-T TGCAAATCGTGGGTATGTGT-3 | ||||
| icaC | 5-CTTGGGTATTTGCACGCATT-3 | AF086783 | 60 | 209 |
| 5-GCAATATCATGCCGACACCT-3 | ||||
| fnbA | 5-AAATTGGGAGCAGCATCAGT-3 | X95848.1 | 60 | 121 |
| 5-GCAGCTGAATTCCCATTTTC-3 | ||||
| fnbB | 5-ACGCTCAAGGCGACGGCAAAG-3 | X62992.1 | 60 | 197 |
| 5-ACCTTCTGCATGACCTTCTGCACCT-3 | ||||
| clfA | 5-ACCCAGGTTCAGATTCTGGCAGCG-3 | Z18852.1 | 60 | 165 |
| 5-TCGCTGAGTCGGAATCGCTTGCT-3 | ||||
| clfB | 5-AACTCCAGGGCCGCCGGTTG-3 | AJ224764.1 | 60 | 159 |
| 5-CCTGAGTCGCTGTCTGAGCCTGAG-3 | ||||
| fib | 5-CGTCAACAGCAGATGCGAGCG-3 | X72014.1 | 60 | 239 |
| 5-TGCATCAGTTTTCGCTGCTGGTTT-3 | ||||
| ebps | 5-GGTGCAGCTGGTGCAATGGGTGT-3 | U48826.2 | 60 | 191 |
| 5-GCTGCGCCTCCAGCCAAACCT-3 | ||||
| eno | 5-TGCCGTAGGTGACGAAGGTGGTT-3 | AF065394.1 | 60 | 195 |
| 5-GCACCGTGTTCGCCTTCGAACT-3 | ||||
| cna | 5-AATAGAGGCGCCACGACCGT-3 | M81736.1 | 60 | 156 |
| 5-GTGCCTTCCCAAACCTTTTGAGCA-3 | ||||
| 16S rRNA | 5-GGGACCCGCACAAGCGGTGG-3 | L37597.1 | 60 | 191 |
| 5-GGGTTGCGCTCGTTGCGGGA-3 |
fnbA and B: fibronectin binding proteins A and B, clfA and B: clumping factors A and B, fib: fibrinogen binding protein, eno: laminin binding protein, cna: collagen binding protein, ebps: elastin binding protein, icaADBC: intercellular adhesion biofilm required genes, 16S rRNA (housekeeping gene), * Optimized PCR annealing temperature program for all the genes at 60 °C.