Figure A4.

Synonymous codon ratios are the ratio between the number of a given codon divided by the total number of codon coding for the same amino acid. By sorting this ratio in blocks of synonymous codons, this heatmap illustrates the preferential codons for each amino acid for each dataset across all genes. A number of codon usage bias are consistent across most genes and datasets. For instance, GCT is preferentially used for Ala (Alanine) and GTT for Val (Valine). On the whole, there seem to be less of a preferential codon use for bat, especially in longer genes or when multiple genes are accounted for, as per indicated by the higher frequency of more evenly distributed codon within each amino acid (i.e., for the bat dataset, the heatmap colours are of a similar level within each amino acid).