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. 2021 Jan 1;7(1):21. doi: 10.3390/jof7010021

Figure 2.

Figure 2

P. lutzii interaction with macrophages assessed by flow cytometry. P. lutzii yeast cells were grown in the presence of BPS, hemoglobin or FeSO4 and incubated for 4 h with RAW 264.7 macrophages. BSA was used as control. Methanol fixed, non-permeabilized cells were incubated with 100 µg/mL of Congo Red. The amount of P. lutzii cells that interacted with macrophages was assessed by flow cytometry using the instrument Guava® easyCyte (MERK). Proportion test was used for statistical comparison between the conditions analyzed. (*) denotes a statistically significant difference.