Figure 3.

Analysis of hemoglobin influence on HSP30 protein expression at the cell surface of P. lutzii. Yeast cells were grown in the presence or absence of hemoglobin. Methanol fixed, non-permeabilized cells were incubated with anti-HSP30 antibodies or with pre immune serum for control. The pictures were taken in bright field and at 450/490 nm of fluorescein isothiocyanate (FITC) probe. All representative pictures were taken using an Axioscope microscope (Carl Zeiss AG, Berlin, Germany) and magnified 400×.