Table 1.
Up-regulated proteins in Fraction 1 (F1) of the cell wall predicted as secreted, following Paracoccidioides lutzii yeast cells exposition to hemoglobin and bathophenanthrolinedisulfonic acid (BPS) for 48 h.
| Accession a | Description b | Score c | Expression LevelsRatio (Hb/BPS) d | SignalP e | SecretomeP f |
|---|---|---|---|---|---|
| PAAG_00871 | 30 kDa heat shock protein (HSP30) • | 754.8 | * | - | 0.786 |
| PAAG_08955 | 40S ribosomal protein S1 | 672.2 | 1.62 | - | 0.720 |
| PAAG_01413 | 40S ribosomal protein S17 | 1850.1 | * | - | 0.738 |
| PAAG_03322 | 40S ribosomal protein S20 | 1303.5 | 1.63 | - | 0.750 |
| PAAG_07847 | 40S ribosomal protein S26E | 409.6 | * | - | 0.613 |
| PAAG_00468 | 4-aminobutyrate aminotransferase | 977.2 | 1.75 | - | 0.601 |
| PAAG_00430 | 60S ribosomal protein L2 | 360.1 | 1.55 | - | 0.853 |
| PAAG_00952 | 60S ribosomal protein L20 | 1022.6 | 1.67 | - | 0.712 |
| PAAG_07786 | Acetyl-CoA acetyltransferase | 1043.1 | 4.10 | - | 0.655 |
| PAAG_05117 | Aspartate-tRNA (Asn) ligase | 565.1 | 5.53 | - | 0.609 |
| PAAG_04838 | ATP synthase subunit 4, mitochondrial | 663.4 | 1.51 | - | 0.781 |
| PAAG_04511 | ATP-dependent RNA helicase SUB2 | 2259.1 | 1.60 | - | 0.722 |
| PAAG_03701 | BAR domain-containing protein | 844.8 | 2.48 | - | 0.614 |
| PAAG_01262 | Chaperone DnaK | 2982.3 | 1.57 | 0.864 | - |
| PAAG_03292 | Cytochrome c peroxidase, mitochondrial • | 3045.4 | 1.60 | - | 0.809 |
| PAAG_00173 | Electron transfer flavoprotein subunit alpha | 465.5 | 1.88 | - | 0.642 |
| PAAG_00850 | Glutamine-fructose-6-phosphate transaminase (isomerizing) | 1305.8 | 1.65 | - | 0.693 |
| PAAG_00126 | Histone H4.2 | 4337.0 | 1.62 | 0.792 | - |
| PAAG_00053 | Malate dehydrogenase, NAD-dependent | 1037.2 | 1.62 | - | 0.651 |
| PAAG_00481 | Membrane biogenesis protein Yop1 | 922.3 | 1.93 | - | 0.902 |
| PAAG_01861 | Membrane-associated progesterone receptor component 1 | 443.0 | * | - | 0.735 |
| PAAG_12076 | NAD(P)H:quinone oxidoreductase, type IV • | 1770.3 | 1.55 | 0.718 | - |
| PAAG_05735 | NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial | 668.4 | 2.05 | - | 0.675 |
| PAAG_01321 | Oxidoreductase 2-nitropropane dioxygenase family • | 2254.9 | 1.79 | - | 0.707 |
| PAAG_00739 | Peptidyl-prolyl cis-trans isomerase B | 583.7 | 1.65 | 0.641 | - |
| PAAG_08082 | Plasma membrane ATPase | 738.9 | 1.93 | - | 0.712 |
| PAAG_07957 | Pre-mRNA splicing factor • | 539.9 | 2.69 | - | 0.801 |
| PAAG_11504 | Protein disulfide-isomerase domain • | 364.5 | * | - | 0.783 |
| PAAG_02769 | Pyruvate dehydrogenase protein X component • | 325.5 | * | - | 0.685 |
| PAAG_00417 | Succinyl-CoA ligase subunit alpha | 1400.3 | 1.88 | - | 0.624 |
| PAAG_02921 | Translation elongation factor Tu | 990.0 | 1.70 | - | 0.773 |
| PAAG_04901 | Ubiquitin-conjugating enzyme | 555.9 | 1.79 | - | 0.883 |
| PAAG_12424 | Voltage-dependent anion channel protein 1 | 2827.7 | 1.54 | - | 0.761 |
a Protein accession number in NCBI, available at https://www.ncbi.nlm.nih.gov/protein. b Description of the protein in the Paracoccidioides spp. databank, available in https://www.uniprot.org/uniprot/?query=paracoccidioides+lutzii&sort=score. c Score of the quality of protein identification. d Ratio between quantification of proteins identified in hemoglobin/iron deprivation. Values ≥ 1.5 indicate upregulated proteins; (*) indicates that the protein was identified only upon hemoglobin treatment. e Prediction of signal peptide presence; score ≥ 0.45; prediction performed by SignalP 4.1 available at http://www.cbs.dtu.dk/services/SignalP/. (-) indicates that the signal peptide was not identified. f Prediction of protein secretion by non-classical pathways; score 0.6; prediction performed by SecretomeP 2.0 available at http://www.cbs.dtu.dk/services/SecretomeP/. (-) indicates that the protein was not predicted as secreted by non-classical pathways. • Prediction of adhesin function; score ≥−0.8; predicted by the FaaPred tool available at http://bioinfo.icgeb.res.in/faap/.