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. 2020 Dec 23;10(1):8. doi: 10.3390/antiox10010008

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemistry (IHC) of collagen type II (Col II) and collagen type X (Col X) in the articular cartilage of control, osteoarthritis (OA), and OA+EGCG joints. (a) The representative micrographs of IHC analysis for Col II and Col X. (b) The quantitative analysis of immunostained Col II. (c) The quantitative analysis of immunostained Col X. The immunostained Col II was reduced and the immunostained Col X was enhanced after anterior cruciate ligament transection (ACLT). There was significantly more staining of Col II and less staining of Col X in the EGCG-treated rats compared with ACLT rats. (** p < 0.01 OA vs. control; ## p < 0.01 OA vs. EGCG).