. 2021 Jan 1;9(1):26. doi: 10.3390/biomedicines9010026

Table 1.

Inhibitory levels of different antibiotics on functions of Primary Human Osteoblasts (PHO), MG63 and HeLa cell lines (adapted and reprinted with permission from Duewelhenke et al. [23]).

Antibiotic	Mean Inhibitory Concentration (μg/mL) for 20% Inhibition (IC₂₀) and 50% Inhibition (IC₅₀) of Proliferation and Metabolic Activity in Different Cell Types (Highest Concentration Tested Was 400 μg/mL)
Antibiotic	IC₂₀PHO	IC₅₀PHO	IC₂₀MG63	IC₅₀ MG63	IC₂₀ HeLa	IC₅₀ HeLa
Penicillin G	No effect	No effect	No effect	No effect	No effect	No effect
Flucloxacillin	No effect	No effect	No effect	No effect	No effect	No effect
Amoxicillin	No effect	No effect	No effect	No effect	No effect	No effect
Cefazolin	380, >400	>400, >400	230, 400	>400, >400	270, >400	>400, >400
Vancomycin	No effect	No effect	No effect	No effect	No effect	No effect
Fosfomycin	No effect	No effect	No effect	No effect	No effect	No effect
Gentamicin	No effect	No effect	No effect	No effect	No effect	No effect
Streptomycin	No effect	No effect	No effect	No effect	No effect	No effect
Tobramycin	No effect	No effect	No effect	No effect	No effect	No effect
Ciprofloxacin	70, 260	170, >400	80, 60	160, 150	100, 70	290, 120
Moxifloxacin	80, 190	160, >400	110, 30	230, 170	90, 40	320, 110
Tetracycline	60, ÷	180, ÷	60, ÷	180, ÷	200, ÷	>400, ÷
Rifampin	30, ÷	130, ÷	120, ÷	240, ÷	180, ÷	270, ÷
Clindamycin	40, 340	150, >400	160, 200	250, >400	230, 80	>400, 200
Lincomycin	No effect	No effect	No effect	No effect	No effect	No effect
Erythromycin	30, 210	180, >400	130, 180	300, 310	210, 110	>400, 170
Roxithromycin	20, 110	70, 210	50, 50	180, 110	100, 30	160, 90
Azithromycin	20, 80	25, 160	30, 70	190, 180	110, 50	240, 160
Chloramphenicol	60, 260	230, >400	200, 110	>400, 400	340, 100	>400, 340
Linezolid	240, >400	>400, >400	No effect, 250	No effect, >400	No effect, 300	No effect, >400

÷ ICs not determined; “No effect” means no cytotoxicity was measured after 48 hours of treatment at the maximum 400 µg/mL dose.