Table 1.
Real-time PCR primer sequence.
| Genes | Accession | Direction | Sequences (5′–3′) | Size |
|---|---|---|---|---|
| Apo AI | NM_214398.1 | Forward | CGTGTATGTGGATGCGATCAAA | 104 |
| Reverse | CCCAGTTGTCCAGGAGTTTCAG | |||
| Apo AIV | AJ222966.1 | Forward | TCAACACCCAGGTTCAGCAG | 121 |
| Reverse | AACTCATCCGCATAGGGTGC | |||
| Apo B48 | AH001207.2 | Forward | GAGGTAACTGTGCCTGCATA | 436 |
| Reverse | CTGCACTAAGGTCACGATGT | |||
| GPR120 | HQ662564.1 | Forward | CTCTTCCTGCTCATGATCTCCTTC | 178 |
| Reverse | GTGACATGTTGTAGAGAATGGGGTT | |||
| FATP4 | XM_003353676.1 | Forward | AGCCGCATCCTGTCCTTT | 213 |
| Reverse | GACATCCTTGGCGATCTTTT | |||
| CD36 | DQ192230.1 | Forward | GGACTCAT TGCTGGTGCTGT | 169 |
| Reverse | GTCTGTAAACTTCCGTGCCTGT | |||
| I-FABP | NM_001031780.1 | Forward | GAGCTTGGTGTCACTTTTAACTAC | 192 |
| Reverse | CCTCTTGGCTTCTACTCCTTCATA | |||
| IL-FABP | NM_214215.2 | Forward | GGAGGTCTCCACTGTCGG | 115 |
| Reverse | CTGTTACACCAGGTTTATTTG | |||
| L-FABP | NM_001004046.2 | Forward | GAAGGGGAAGGACATCA | 138 |
| Reverse | CAGTCAGGGTCTCCATCTCACA | |||
| Claudin | NM_001244539.1 | Forward | TCTTTCTTATTTCAGGTCTGGCTATCT | 247 |
| Reverse | CCACTGGAAGGCGAAGGTTTT | |||
| Occludin | NM_001163647.2 | Forward | ACTGGCGGCGAGTCCTGCGACGAGC | 244 |
| Reverse | TATTGTATTCATCAGCAGCAGCCAT | |||
| ZO-1 | XM_021098856.1 | Forward | AAAGCCCTAAGTTCAATCACAATCT | 253 |
| Reverse | TCCTCATCTTCATCATCTTCTACAG | |||
| pBD-1 | NM_213838.1 | Forward | TGCCACAGGTGCCGATCT | 81 |
| Reverse | CTGTTAGCTGCTTAAGGAATAAAGGC | |||
| pBD-2 | NM_214442.2 | Forward | CCAGAGAGGTCCGACACTACA | 168 |
| Reverse | GGTCCCTTCAATCCTTGTAGGTGAA | |||
| pBD-3 | XM_021074698.1 | Forward | ACCAAGCACGCCTTCCTATC | 236 |
| Reverse | GCATTTTCGGCCACTCACAG | |||
| pBD-114 | NM_001129973.1 | Forward | TGTACCTTGGTGGATCCTGAACGA | 240 |
| Reverse | CGCCCTCTGAATGCAGCATATCTT | |||
| pBD-129 | NM_001129975.1 | Forward | CAAAGACCACTGTGCCGTGAATGA | 131 |
| Reverse | TTGATGCTGGCGAAAGGGTTGGTA | |||
| β-actin | 397563 | Forward | TGCGGGACATCAAGGAGAAG | 176 |
| Reverse | AGTTGAAGGTGGTCTCGTGG |
Apo AI, apolipoprotein AI; Apo AIV, apolipoprotein AIV; Apo B48, apolipoprotein B48; FATP4, fatty acid transport protein 4; CD36, fatty acid translocase; GPR120, G protein coupled receptors 120; I-FABP, intestinal fatty acid binding protein; IL-FABP, ileum fatty acid binding protein; L-FABP, liver fatty acid binding protein; pBD-1, porcine β-defensin 1; pBD-2, porcine β-defensin 2; pBD-3, porcine β-defensin 3; pBD-114, porcine β-defensin 114; pBD-129, porcine β-defensin 129.