Table 1.

Primer pairs used for PCR amplification.

Gene Target	Primer Pairs	Primer Sequence (5′-3′)	Filarial Species	Product Size (bp)
ITS1	ITS1-F ITS1-R	GGT GAA CCT GCG GAA GGA TC GAG TTA CGC AGA CGT TAA GCG	W. bancrofti	482
			B. malayi	504
			B. pahangi	510
			D. immitis	595
			D. repens	602
5.8S-ITS2-28S	DIDR-F1 DIDR-R1	AGT GCG AAT TGC AGA CGC ATT GAG AGC GGG TAA TCA CGA CTG AGT TGA	D. immitis	542
			A. reconditum	578
			D. repens	484
			A. dracunculoides	584
			B. pahangi	664
			B. malayi	615
COI	DI COI -F1 DI COI-R1	AGT GTA GAG GGT CAG CCT GAG TTA ACA GGC ACT GAC AAT ACC AAT	D. immitis	203