Table 2.
Effect of metformin (500 mg/kg bwt) and/or chitosan-stabilized selenium nanoparticles (2 mg Se/kg bwt) on lesion scoring of testicular tissues of HFD/STZ-diabetic rats.
| Lesion | Control | HFD/STZ | HFD/STZ+MF | HFD/STZ+CH-SeNPs | HFD/STZ+MF+CH-SeNps |
|---|---|---|---|---|---|
| Spermatogonial cells/ST | 66.90 a± 1.16 | 48.50 c ± 0.96 | 59.80 b ± 1.16 | 49.90 c ± 0.92 | 62.50 b ± 0.86 |
| Spermatocytes/ST | 147.30 a ± 1.46 | 105.10 d ± 1.37 | 128.30 c ± 3.25 | 111.50 e ± 1.42 | 140.70 b ± 1.80 |
| Spermatid/ST | 221.30 a ± 5.14 | 170.40 c ± 2.97 | 199.30 b ± 1.04 | 174.70 c ± 2.41 | 212.60 a ± 3.69 |
| Sertoli cells/ST | 30.50 a ± 0.48 | 26.20 b ± 0.88 | 29.30 a ± 0.70 | 26.60 b ± 0.78 | 29.80 a ± 0.59 |
| Leydig cells/intertubular area | 13.60 a ± 0.45 | 10.40 b ± 0.45 | 12.30 a ± 0.52 | 10.80 b ± 0.39 | 12.60 a ± 0.43 |
| Height of germinal epithelium | 83.22 a ± 1.70 | 58.09 c ± 1.94 | 77.04 b ± 2.50 | 55.55 c ± 1.83 | 80.16 ab ± 2.02 |
| Numbers of STs/10X | 14.20 b ± 0.36 | 18.20 a ± 0.33 | 14.80 b ± 0.44 | 17.20 a ± 0.47 | 14.50 b ±0.22 |
| Mean diameter of ST | 255.36 a ± 1.37 | 224.74 b ± 4.67 | 248.23 a ± 1.63 | 229.27 b ± 1.87 | 252.93 a ± 1.06 |
| STs with vacuolated germinal epithelium | 0.00 d ± 0.00 | 7.38 a ± 0.32 | 4.04 c ± 0.23 | 5.57 b ± 0.33 | 3.55 c ± 0.28 |
| STs with desquamated germinal epithelium | 0.00 d ± 0.00 | 20.10 a ± 1.32 | 11.14 b ± 1.23 | 17.10 a ± 1.77 | 3.74 c ± 0.42 |
| STs with depleted germ cells | 0.00 e ± 0.00 | 15.88 a ±1.34 | 7.36 c ± 0.23 | 11.91 b ± 0.75 | 3.58 d ± 0.33 |
| STs with necrotic and or complete loss of germinal epithelium | 0.00 e ± 0.00 | 4.83 a ± 0.43 | 2.05 c ± 0.17 | 3.53 b ± 0.22 | 1.10 d ± 0.10 |
| STs with multinucleated giant cell formation | 0.00 b ± 0.00 | 3.07 a ± 1.19 | 1.00 b ± 0.12 | 1.31 b ± 0.07 | 0.40 b ± 0.17 |
| STs with spermatid retention | 0.00 c ± 0.00 | 0.86 ab ± 0.31 | 0.75 abc ± 0.33 | 1.09 a ± 0.31 | 0.25 bc ± 0.17 |
| STs with uneven, or redundant or broken basal lamina | 0.00 e ± 0.00 | 14.47 a ± 1.01 | 7.40 c ±0.43 | 10.94 b ± 1.00 | 3.06 d ± 0.45 |
| Interstitial leukocytic infiltration | 0.00 b ± 0.00 | 10.66 a ± 1.71 | 3.00 b ± 0.92 | 9.00 a ± 1.58 | 1.00 b ± 0.51 |
| Interstitial edema | 0.00 b ±0.00 | 12.67 a ± 1.47 | 2.33 b ± 1.00 | 10.67 a ± 2.42 | 3.33 b ± 1.99 |
| Interstitial congestion | 0.00 d ± 0.00 | 15.33 a ± 1.87 | 4.33 c ± 0.71 | 9.97 b ± 1.78 | 2.66 c d ± 0.83 |
| Interstitial hemorrhage | 0.00 b ± 0.00 | 2.33 a ± 0.71 | 1.00 b ± 0.51 | 0.67 b ± 0.44 | 0.33 b ± 0.33 |
Values are mean ± SE. Means within the same row carrying different superscripts (a–e) are significantly different at p < 0.05.