Table 5.
Lipase, α-amylase, and α-glucosidase inhibitory activity [IC50 (μg/mL)] of Hypochaeris and Hyoseris extracts.
| Sample | Lipase | α-Amylase | α-Glucosidase |
|---|---|---|---|
| Hypochaeris leavigata (HL) | |||
| HL1 | 58.2 ± 1.5 c | 42.7 ± 1.3 c | 31.3 ± 1.3 c |
| HL2 | 85.0 ± 1.8 a | 94.9 ± 2.0 a | 81.6 ± 1.8 a |
| HL3 | 66.4 ± 1.7 b | 56.8 ± 1.5 b | 62.3 ± 1.6 b |
| Sign. | ** | ** | ** |
| Hypochaeris radicata (HR) | |||
| HR1 | 52.4 ± 1.5 c | 56.9 ± 1.4 c | 37.6 ± 1.3 c |
| HR2 | 81.4 ± 1.7 a | 112.9 ± 2.1 a | 118.8 ± 2.3 a |
| HR3 | 62.8 ± 1.7 b | 90.1 ± 2.0 b | 43.9 ± 1.4 b |
| Sign. | ** | ** | ** |
| Hyoseris radiata (HRA) | |||
| HRA1 | 39.8 ± 1.4 c | 79.4 ± 1.7 c | 41.9 ± 1.4 c |
| HRA2 | 85.7 ± 1.8 a | 99.1 ± 1.9 a | 84.5 ± 1.8 a |
| HRA3 | 65.7 ± 1.7 b | 83.4 ± 1.8 b | 51.9 ± 1.5 b |
| Sign. | ** | ** | ** |
| Hyoseris lucida subsp. taurina (HT) | |||
| HT1 | 73.5 ± 1.7 c | 74.0 ± 1.7 c | 63.1 ± 1.6 c |
| HT2 | 89.6 ± 1.9 a | 94.2 ± 1.9 a | 94.9 ± 1.9 a |
| HT3 | 78.8 ± 1.8 b | 82.8 ± 1.6 b | 74.4 ± 1.7 b |
| Sign. | ** | ** | ** |
Data are expressed to mean ± Standard Deviation (SD) (n = 3). 1: Extract fresh plant materials; 2: Extract plant materials after blanching; 3: Blanching water extract. Orlistat used as positive control in lipase test (IC50 = 37.4 ± 1.0). Acarbose used as positive control in α-amylase and α-glucosidase tests (IC50 = 50.1 ± 1.3 and 35.5 ± 0.9 respectively for α-amylase α-glucosidase). One-way ANOVA followed by Tukey’s multiple range test was applied for statistical analysis. Different letters in the same column are significantly different ** at p < 0.01.