Figure 3.
TEER measurement circuit hypothesis and Transwell® plate measurement. (A) photograph of the actual Transwell® plate and how TEER measurement is done. (B) electrode arrangement of EVOM2, (C) TEER measurement circuit; the resistances of cell growth medium and membrane are denoted by Rmedium and Rmembrane, respectively. Rmembrane is extremely high; therefore, the parallel connection of the Rmembrane and Ccell can be simplified as Ccell [18]. Celectrodes is the capacitance of the electrode for resistance measurement, and RTEER is the resistance of the paracellular tight-junction channel, which is the primary measurement target in this experiment.