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Procedure for obtaining samples from the Mueller–Hinton agar growth medium to establish the concentration of PTS and PTSO reached on it via the gas phase of both compounds by HPLC-UV. For each organosulfur compound and concentration, after incubation for 24 h at 36 ± 1 °C, samples with a diameter of 7.5 mm were extracted from the center of the plate to the periphery in order to determine the concentration of PTS and PTSO reached on the agar as a consequence of its evaporation.
