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. 2020 Dec 24;9(1):30. doi: 10.3390/microorganisms9010030

Figure 3.

Figure 3

(A) Consumption kinetics of methanogenesis substrates during the weeks of incubation of the substrate-amended slurry performed with the PENF sample; (B) Consumption kinetics of some metabolic products (methane, ammonia, propionate) during the weeks of incubation of the substrate-amended slurry performed with the PENF sample. Concentrations of propionate are indicated on the right vertical axis of the figure and other microbial products (CH4 and NH4+) are indicate on the left vertical axis. The vertical red line indicates a novel addition of trimethylamine (TMA), methanol and H2/CO2 at T6 to stimulate methanogenesis; (C) Trends in microbial diversity revealed by metabarcoding; (D) Methanomassiliicoccales abundances determined by qPCR (logarithmic scale). Methanomassiliicoccales could not be quantified by qPCR in 4 samples (T0, T1, T6, and T7). Average copy numbers are given on the chart bars. Renewal of methanogenesis substrates (H2, CO2, MeOH, TMA) after six weeks of incubation is represented by the vertical red lines. Peptostreptococcales-T.: Peptostreptococcales-Tissierellales.