Table 1.

Generation of amplicons using primers and conditions outlined (Australian Genome Research Facility, AGRF, Melbourne, Australia).

Target	Cycle	Initial	Disassociate	Anneal	Extension	Finish
16S: V3–V4	29	95 °C for 7 min	94 °C for 30 s	50 °C for 60 s	72 °C for 60 s	72 °C for 7 min
Target	341F-806R
Forward Primer(341F)	CCTAYGGGRBGCASCAG
ReversePrimer(806R)	GGACTACNNGGGTATCTAAT