Figure 3.

Multiple sequence alignment of a-type domains of tomato PDI proteins alongside those of a classic human PDI. The thioredoxin-like domains in tomato PDIs were annotated by the SMART webtool, and aligned using Clustal Omega. The elements of the secondary structure are indicated by blue (α-helices) and black (β-sheets) bars above the alignment. The red arrows indicate the Glu-Lys charged pair located near the active site, a green arrow indicates the conserved Arg (R) between β5 and α4 of each catalytic domain, and a blue arrow indicates the cis Pro (P) near each active site. The –CXXC– catalytic sites are marked by a green box.