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. 2020 Dec 30;13(1):46. doi: 10.3390/v13010046

Figure 1.

Figure 1

P-selectin glycoprotein ligand-1 (PSGL-1) inactivates the infectivity of SARS-CoV and SARS-CoV2 pseudoviruses. (A) Schematic of the assembly of lentiviral particles pseudotyped with the S proteins of SARS-CoV and SARS-CoV-2. (B,C) Effects of PSGL-1 on viral release. A PSGL-1 expression vector or control empty vector was co-transfected with the lentivirus packaging construct and a GFP or luciferase reporter plasmid, and viral release was quantified at 72 h post-transfection by HIV-1 p24 ELISA. (D) The infectivity of SARS-CoV pseudotyped virions was quantified by infecting Vero E6 cells and measuring GFP expression at 72 h post-infection. The percentages of GFP+ cells are shown. (E,F) The infectivity of the SARS-CoV-2 pseudotyped virions was quantified by infecting Vero E6 (E) and Calu-3 cells (F). Luciferase activity in infected cells was quantified at 72 h post-infection.