Figure 5.

PDIA4 is not required for astrovirus replication when the entry step is bypassed by transfection of the viral genomic RNA. Caco-2 cells were transfected with 200 ng of total RNA extracted from HAstV-8 infected cells that were previously (A) treated or not with the indicated concentrations of 16F16 or (B) transfected either with the PDIA4 siRNA or with an irrelevant siRNA for 72 h. After 24 h post-transfection, the infectivity was determined as described in Material and Methods. Virus infectivity is expressed as the percentage of infected cells obtained in the control cells treated with DMSO or that were transfected with the irrelevant siRNA, which were taken as 100%, respectively. The arithmetic means ± SEM from three independent experiments performed in duplicate is shown; ns, non-significant.